Recombinant Antarctomyces psychrotrophicus Antifreeze protein (X10S), partial

SKU: P86268

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P86268

Gene Names: N/A

Alternative Name(s): AnpAFP

Abbreviation: Recombinant Antarctomyces psychrotrophicus Antifreeze protein (X10S), partial

Organism: Antarctomyces psychrotrophicus

Source: E.coli

Expression Region: 1-20aa(X10S)

Protein Length: Partial

Tag Info: N-terminal 6xHis-KSI-tagged

Target Protein Sequence: AGLDLGAASSFGALAFEGVA

MW: 17.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Antifreeze proteins bind to the surface of ice crystals and inhibit the growth of these crystals, this inhibition causes thermal hysteresis. Causes the shape of ice crystals to change from hexagonal to a bipyramidal shape with rugged facets. Inhibits recrystallization of ice crystals.

Reference:

Function: