Rat TRACP-5b(TartrateResistant Acid Phosphatase 5b) ELISA Kit

SKU: ELK9632

Size:96T

Reactivity:Rat

Alternative Names:ACP5;TRAP5b;SPENCDI

Assay Type:Sandwich

Sensitivity:0.36 ng/mL

Standard:40 ng/mL

Detection range:0.63-40 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Immunology;Cardiovascular

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: