Rat TNFa(Tumor Necrosis Factor Alpha)（For cellular supernatant） ELISA Kit

SKU: ELK1396C

Size:96T

Reactivity:Rat

Alternative Names:TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Assay Type:Sandwich

Sensitivity:6.3 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:Cell culture supernates,cell lysates,tissue homogenates

Assay length:3.5h

Research Area:Cytokine;Tumor immunity;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P16599