Rat sCD14(SolubleCluster of Differentiation 14) ELISA Kit

SKU: ELK11355

Size:96T

Reactivity:Rat

Alternative Names:CD14 Antigen; Monocyte Differentiation Antigen CD14

Assay Type:Sandwich

Sensitivity:0.96 ng/mL

Standard:100 ng/mL

Detection range:1.56-100 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Immunology;Stem Cells;Kits/ Lysates/ Other

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sCD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sCD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sCD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sCD14 in the samples is then determined by comparing the OD of the samples to the standard curve.

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