Rat NPFF(Neuropeptide FF) ELISA Kit

SKU: ELK10833

Size:96T

Reactivity:Rat

Alternative Names:FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF

Assay Type:Competitive Inhibition

Sensitivity:5.9 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Neuro science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPFF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9WVA9