Rat iPLA2(Phospholipase A2, Calcium Independent) ELISA Kit

SKU: ELK5029

Size:96T

Reactivity:Rat

Alternative Names:PLA2G6; CaI-PLA2; GVI; INAD1; PNPLA9; Ca2+ Independent PLA2; 85 kDa Calcium-Independent Phospholipase A2; Phospholipase A2,group VI(Cytosolic,Calcium-Independent)

Assay Type:Sandwich

Sensitivity:0.135 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Enzyme & Kinase;Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat iPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat iPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat iPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat iPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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