Rat Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta, IKBKB ELISA Kit

SKU: E2113Ra

Size:96 Tests-1 Kit

Background:Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation (By similarity). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (By similarity).

Research_area:Others

Applications:ELISA

Target_protein:IKBKB

Reactivity:Rat

Assay type:Sandwich

Sensitivity:10.91ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9QY78

MASS(Da):86,866

GeneID:84351

Synonyms:I-kappa-B kinase 2;I-kappa-B-kinase beta;IKBKB;IKK2;IKK-B;IKK-beta;Inhibitor of nuclear factor kappa-B kinase subunit beta;NFKBIKB;Nuclear factor NF-kappa-B inhibitor kinase beta;Serine/threonine protein kinase IKBKB

Gene names:IKBKB

Standard solution:4800ng/L