Rat Cytochrome P450 1A2, CYP1A2 ELISA Kit

SKU: E3303Ra

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.

Research_area:Others

Applications:ELISA

Target_protein:CYP1A2

Reactivity:Rat

Assay type:Sandwich

Sensitivity:10.24ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 1A2

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P04799

MASS(Da):58,259

GeneID:24297

Synonyms:Cholesterol 25-hydroxylase;CYP1A2;CYPD45;CYPIA2;Cytochrome P-448;Cytochrome P450 1A2;Cytochrome P450-D;Cytochrome P-450d;Hydroperoxy icosatetraenoate dehydratase;P-450d;RATCYPD45

Gene names:Cyp1a2

Standard solution:4800ng/L