Rat CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit

SKU: ELK5634

Size:96T

Reactivity:Rat

Alternative Names:CYSLT2; GPCR; CYSLT2R; HG57; HPN321; HGPCR21; G-protein coupled receptor GPCR21; G-protein coupled receptor HG57

Assay Type:Sandwich

Sensitivity:0.107 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Signal transduction;Infection immunity;Rheumatology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYSLTR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYSLTR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYSLTR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYSLTR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q924T9