Rat CXCL16(Chemokine C-X-C-Motif Ligand 16) Microsample ELISA Kit

SKU: ELK1706MS

Size:96T

Reactivity:Rat

Alternative Names:SR-PSOX; CXCLG16; SRPSOX; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; Small-inducible cytokine B16

Assay Type:Sandwich

Sensitivity:0.117 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length:3.5h

Research Area:Cytokine;Tumor immunity;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CXCL16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CXCL16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CXCL16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CXCL16 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q6AXU5