Rat CORO1A(Coronin 1A) ELISA Kit

SKU: ELK6621

Size:96T

Reactivity:Rat

Alternative Names:CLABP; p57; CLIPINA; HCORO1; TACO; Coronin-1; Coronin,Actin-Binding Protein,1A; Coronin-like protein A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein

Assay Type:Sandwich

Sensitivity:0.114 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Tissue homogenates, cell lysates and other biological fluids.

Assay length:3.5h

Research Area:Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CORO1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CORO1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CORO1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CORO1A in the samples is then determined by comparing the OD of the samples to the standard curve.

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