Rat CD30L(Cluster Of Differentiation 30 Ligand) Microsample ELISA Kit

SKU: ELK7637MS

Size:96T

Reactivity:Rat

Alternative Names:CD153; TNFSF8; CD30-L; Tumor Necrosis Factor Ligand Superfamily Member 8

Assay Type:Sandwich

Sensitivity:49 pg/mL

Standard:8000 pg/mL

Detection range:125-8000 pg/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD30L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD30L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD30L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD30L in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P32972