Rat Caspase-1, CASP1 ELISA Kit

SKU: E1897Ra

Size:96 Tests-1 Kit

Background:Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.

Research_area:Cell Biology; Cancer; Metabolism

Applications:ELISA

Target_protein:CASP1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:1.53ng/L

Detection range:3-900ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Caspase-1

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P43527

MASS(Da):45,576

GeneID:25166

Synonyms:CASP1;CASP-1;Caspase-1;Caspase-1 subunit p10];Caspase-1 subunit p20;Ice;IL-1 beta-converting enzyme;Il1bc;IL-1BC;Interleukin-1 beta convertase;Interleukin-1 beta-converting enzyme;p45

Gene names:Casp1

Standard solution:960ng/L