Rat Acly(ATP-citRate synthase) ELISA Kit

SKU: ELK8567

Size:96T

Reactivity:Rat

Alternative Names:ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP

Assay Type:Sandwich

Sensitivity:0.267 ng/mL

Standard:40 ng/mL

Detection range:0.63-40 ng/mL

Sample type:tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Metabonomics

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Acly. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Acly. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Acly, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Acly in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P16638