Rat 3-nitrotyrosine (3-NT) ELISA Kit

SKU: AE22924RA

Size:96T. Other sizes are also available. Please Inquire.

Species Reactivity:Rat (Rattus norvegicus)

UniProt:N/A

Abbreviation:3-NT

Alternative Names:N/A

Application:ELISA

Range:0.156-10 ng/mL

Sensitivity:0.039 ng/mL

Intra-AssayCV:≤5.2%

Inter-AssayCV:≤7.1%

Recovery:1,02

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate 3-NT in samples. An antibody specific for 3-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any3-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 3-NT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 3-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).