RAD1 antibody , Cat. # FNab07073

SKU: FNab07073

Size:100ug

Purification:Immunogen affinity purified

Form:liquid

Purity:?95% as determined by SDS-PAGE

Host:Rabbit

Clonality:polyclonal

Clone ID:

Isotype:IgG

Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)

Background:Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER). The 9-1-1 complex stimulates DNA polymerase beta(POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I(LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks(DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

Immunogen:RAD1 homolog(S. pombe)

Synonyms:REC1

Observed MW:32 kDa

Uniprot ID:O60671

Reactivity:Human, Mouse, Rat

Tested Application:ELISA, WB, IHC, IP

Recommended dilution:WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

Gene ID:5810

Research Area:Epigenetics, Metabolism