Purified Rabbit anti-ZSCAN4C Antibody

SKU: DL97280A

Size 1: 100 ul

Synonyms : Gm397; Zinc finger and SCAN domain containing protein 4C

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: M

Applications: WB

Background: Rabbit polyclonal antibody to ZSCAN4C

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide of human ZSCAN4C

WB description: Western blot analysis of cTnI expression in mouse heart (A), rat heart (B) whole cell lysates.

IHC description: Immunohistochemical analysis of cTnI staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of cTnI staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 7137

Gene ID (Mouse): 21954

Gene ID(Rat): 29248

SwissProt (Human): P19429

SwissProt (Mouse): P48787

SwissProt (Rat): P23693

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.