Purified Rabbit anti-ZNF265 Antibody

SKU: DL93964A

Size 1: 100 ul

Synonyms : ZIS; ZNF265; Zinc finger Ran-binding domain-containing protein 2; Zinc finger protein 265; Zinc finger, splicing

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, D, Mk, P, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to ZNF265

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZNF265. The exact sequence is proprietary.

WB description: Western blot analysis of ZNF265 expression in HepG2 (A), MCF7 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of ZNF265 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ZNF265 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 9406

Gene ID (Mouse): 53861

Gene ID(Rat): 58821

SwissProt (Human): O95218

SwissProt (Mouse): Q9R020

SwissProt (Rat): O35986

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.