Purified Rabbit anti-VPS72 Antibody

SKU: DL93948A

Size 1: 100 ul

Synonyms : TCFL1; YL1; Vacuolar protein sorting-associated protein 72 homolog; Protein YL-1; Transcription factor-like 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, B

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to VPS72

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VPS72. The exact sequence is proprietary.

WB description: Western blot analysis of VPS72 expression in NIH3T3 (A), mouse kidney (B), mouse heart (C) whole cell lysates.

IHC description: Immunohistochemical analysis of VPS72 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of VPS72 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 6944

Gene ID (Mouse): 21427

Gene ID(Rat):

SwissProt (Human): Q15906

SwissProt (Mouse): Q62481

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.