Purified Rabbit anti-Vimentin Antibody

SKU: DL91228A

Size 1: 100 ul

Synonyms : Vimentin

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Mk, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Vimentin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Vimentin. The exact sequence is proprietary.

WB description: Western blot analysis of Vimentin expression in MCF7 (A), MDAMB435 (B), KNRK (C), HeLa (D), Raw264.7 (E), PC12 (F) whole cell lysates.

IHC description: Immunohistochemical analysis of Vimentin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Vimentin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 7431

Gene ID (Mouse): 22352

Gene ID(Rat): 81818

SwissProt (Human): P08670

SwissProt (Mouse): P20152

SwissProt (Rat): P31000

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.