Purified Rabbit anti-VDR (pS208) Antibody

SKU: DL93308A

Size 1: 100 ul

Synonyms : NR1I1; Vitamin D3 receptor; VDR; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, Mk

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to VDR (pS208)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VDR (pS208). The exact sequence is proprietary.

WB description: Western blot analysis of VDR (pS208) expression in HeLa (A), HepG2 (B) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of VDR (pS208) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Gene ID(Human): 7421

Gene ID (Mouse): 22337

Gene ID(Rat): 24873

SwissProt (Human): P11473

SwissProt (Mouse): P48281

SwissProt (Rat): P13053

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.