Purified Rabbit anti-UGDH Antibody

SKU: DL97169A

Size 1: 100 ul

Synonyms : UDP-glucose 6-dehydrogenase; UDP-Glc dehydrogenase; UDP-GlcDH; UDPGDH

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to UGDH

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human UGDH

WB description: Western blot analysis of UGDH expression in HepG2 (A), Hela (B) whole cell lysates.

IHC description: Immunohistochemical analysis of UGDH staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of UGDH staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 7358

Gene ID (Mouse): 22235

Gene ID(Rat): 83472

SwissProt (Human): O60701

SwissProt (Mouse): O70475

SwissProt (Rat): O70199

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.