Purified Rabbit anti-TSG101 Antibody

SKU: DL94814A

Size 1: 100 ul

Synonyms : Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to TSG101

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TSG101. The exact sequence is proprietary.

WB description: Western blot analysis of TSG101 expression in Hela (A), mouse brain (B), rat brain (C), PC3 (D), HEK293T (E) whole cell lysates.

IHC description: Immunohistochemical analysis of TSG101 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TSG101 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)

Gene ID(Human): 7251

Gene ID (Mouse): 22088

Gene ID(Rat): 292925

SwissProt (Human): Q99816

SwissProt (Mouse): Q61187

SwissProt (Rat): Q6IRE4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.