Purified Rabbit anti-Surfactant Protein C Antibody

SKU: DL97626A

Size 1: 100 ul

Synonyms : SFTP2; Pulmonary surfactant-associated protein C; SP-C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Surfactant Protein C

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Surfactant Protein C

WB description: Western blot analysis of Surfactant Protein C expression in Jurkat (A), Raji (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Surfactant Protein C staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Surfactant Protein C staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 6440

Gene ID (Mouse):

Gene ID(Rat): 50683

SwissProt (Human): P11686

SwissProt (Mouse): P21841

SwissProt (Rat): P11685

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.