Purified Rabbit anti-SPFH1 Antibody

SKU: DL95345A

Size 1: 100 ul

Synonyms : ERLIN1; C10orf69; KE04; KEO4; SPFH1; Erlin-1; Endoplasmic reticulum lipid raft-associated protein 1; Protein KE04; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; SPFH domain-containing protein 1; ERLIN2; C8orf2; SPFH2; Erlin-2; Endoplasmic reticulum lipid raft-associated protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; SPFH domain-containing protein 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Z

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SPFH1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPFH1. The exact sequence is proprietary.

WB description: Western blot analysis of SPFH1 expression in HepG2 (A), NIH3T3 (B), Hela (C) whole cell lysates.

IHC description: Immunohistochemical analysis of SPFH1 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 10613; 11160

Gene ID (Mouse): 226144; 244373

Gene ID(Rat): 290823

SwissProt (Human): O75477; O94905

SwissProt (Mouse): Q91X78; Q8BFZ9

SwissProt (Rat): B5DEH2

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.