Purified Rabbit anti-SPAK Antibody

SKU: DL94238A

Size 1: 100 ul

Synonyms : SPAK; STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SPAK

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPAK. The exact sequence is proprietary.

WB description: Western blot analysis of SPAK expression in Jurkat (A), HepG2 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of SPAK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 27347

Gene ID (Mouse): 53416

Gene ID(Rat): 54348

SwissProt (Human): Q9UEW8

SwissProt (Mouse): Q9Z1W9

SwissProt (Rat): O88506

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.