Purified Rabbit anti-Sm E Antibody

SKU: DL96087A

Size 1: 100 ul

Synonyms : Small nuclear ribonucleoprotein E; snRNP-E; Sm protein E; Sm-E; SmE

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Sm E

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Sm E

WB description: Western blot analysis of Sm E expression in A549 (A), K562 (B), HepG2 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Sm E staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Sm E staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 6635

Gene ID (Mouse): 20643

Gene ID(Rat):

SwissProt (Human): P62304

SwissProt (Mouse): P62305

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.