Purified Rabbit anti-SHARP1 Antibody

SKU: DL92433A

Size 1: 100 ul

Synonyms : BHLHB3; DEC2; SHARP1; Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SHARP1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SHARP1. The exact sequence is proprietary.

WB description: Western blot analysis of SHARP1 expression in SHSY5Y (A), RAW264.7 (B), PC12 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 79365

Gene ID (Mouse): 79362

Gene ID(Rat):

SwissProt (Human): Q9C0J9

SwissProt (Mouse): Q99PV5

SwissProt (Rat): O35779

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.