Purified Rabbit anti-RAD50 Antibody

SKU: DL91455A

Size 1: 100 ul

Synonyms : DNA repair protein RAD50; hRAD50

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to RAD50

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD50. The exact sequence is proprietary.

WB description: Western blot analysis of RAD50 expression in HeLa (A), K562 (B), Jurkat (C), MCF7 (D), Ramos (E), SW480 (F) whole cell lysates.

IHC description: Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 10111

Gene ID (Mouse):

Gene ID(Rat): 64012

SwissProt (Human): Q92878

SwissProt (Mouse): P70388

SwissProt (Rat): Q9JIL8

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.