Purified Rabbit anti-PU.1 Antibody

SKU: DL93526A

Size 1: 100 ul

Synonyms : Transcription factor PU.1; 31 kDa-transforming protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, P, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PU.1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PU.1. The exact sequence is proprietary.

WB description: Western blot analysis of PU.1 expression in HepG2 (A), TF1 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of PU.1 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PU.1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 6688

Gene ID (Mouse): 20375

Gene ID(Rat): 366126

SwissProt (Human): P17947

SwissProt (Mouse): P17433

SwissProt (Rat): Q6BDS1

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.