Purified Rabbit anti-PIP4K2 alpha Antibody

SKU: DL98200A

Size 1: 100 ul

Synonyms : PIP5K2; PIP5K2A; Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha; 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; Diphosphoinositide kinase 2-alpha; PIP5KIII; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; PI(5)P 4-kinase type II alpha; PIP4KII-alpha; PtdIns(4)P-5-kinase B isoform; PtdIns(4)P-5-kinase C isoform; PtdIns(5)P-4-kinase isoform 2-alpha

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PIP4K2 alpha

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PIP4K2 alpha

WB description: Western blot analysis of PIP4K2 alpha expression in Jurkat (A), MCF7 (B), mouse brain (C), rat brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of PIP4K2 alpha staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PIP4K2 alpha staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 5305

Gene ID (Mouse): 18718

Gene ID(Rat): 116723

SwissProt (Human): P48426

SwissProt (Mouse): O70172

SwissProt (Rat): Q9R0I8

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.