Purified Rabbit anti-pan methyl-lysine Antibody

SKU: DL97982A

Size 1: 100 ul

Synonyms : Pan methyl-lysine

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: All

Applications: WB, IP

Background: Rabbit polyclonal antibody to pan methyl-lysine

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide of methyl-lysine

WB description: Western blot analysis of ACTN2 expression in HeLa (A), mouse kidney (B), H9C2 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of ACTN2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ACTN2 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 88; 89

Gene ID (Mouse): 11472; 11474

Gene ID(Rat):

SwissProt (Human): P35609; Q08043

SwissProt (Mouse): Q9JI91; O88990

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.