Purified Rabbit anti-NFYB Antibody

SKU: DL93968A

Size 1: 100 ul

Synonyms : HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, D, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to NFYB

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFYB. The exact sequence is proprietary.

WB description: Western blot analysis of NFYB expression in A549 (A), HEK293T (B) whole cell lysates.

IHC description: Immunohistochemical analysis of NFYB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 4801

Gene ID (Mouse): 18045

Gene ID(Rat): 25336

SwissProt (Human): P25208

SwissProt (Mouse): P63139

SwissProt (Rat): P63140

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.