Purified Rabbit anti-NEFL Antibody

SKU: DL93476A

Size 1: 100 ul

Synonyms : NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to NEFL

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NEFL. The exact sequence is proprietary.

WB description: Western blot analysis of NEFL expression in Jurkat (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of NEFL staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 4747

Gene ID (Mouse): 18039

Gene ID(Rat): 83613

SwissProt (Human): P07196

SwissProt (Mouse): P08551

SwissProt (Rat): P19527

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.