Purified Rabbit anti-NEDD8 Antibody

SKU: DL90793A

Size 1: 100 ul

Synonyms : NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, Rb

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to NEDD8

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NEDD8. The exact sequence is proprietary.

WB description: Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates.

IHC description: Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 4738

Gene ID (Mouse): 18002

Gene ID(Rat): 25490

SwissProt (Human): Q15843

SwissProt (Mouse): P29595

SwissProt (Rat): Q71UE8

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.