Purified Rabbit anti-NCK1 Antibody

SKU: DL96957A

Size 1: 100 ul

Synonyms : NCK; Cytoplasmic protein NCK1; NCK adaptor protein 1; Nck-1; SH2/SH3 adaptor protein NCK-alpha

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to NCK1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human NCK1

WB description: Western blot analysis of NCK1 expression in Hela (A), mouse lung (B), mouse brain (C) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of NCK1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Gene ID(Human): 4690

Gene ID (Mouse): 17973

Gene ID(Rat):

SwissProt (Human): P16333

SwissProt (Mouse): Q99M51

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.