Purified Rabbit anti-MRG15 Antibody

SKU: DL94071A

Size 1: 100 ul

Synonyms : MRG15; Mortality factor 4-like protein 1; MORF-related gene 15 protein; Protein MSL3-1; Transcription factor-like protein MRG15

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to MRG15

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRG15. The exact sequence is proprietary.

WB description: Western blot analysis of MRG15 expression in K562 (A), Jurkat (B), mouse kidney (C), mouse brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of MRG15 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of MRG15 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 10933

Gene ID (Mouse): 21761

Gene ID(Rat):

SwissProt (Human): Q9UBU8

SwissProt (Mouse): P60762

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.