Purified Rabbit anti-LKB1 Antibody

SKU: DL92329A

Size 1: 100 ul

Synonyms : LKB1; PJS; Serine/threonine-protein kinase STK11; Liver kinase B1; LKB1; hLKB1; Renal carcinoma antigen NY-REN-19

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to LKB1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human LKB1. The exact sequence is proprietary.

WB description: Western blot analysis of LKB1 expression in A431 (A), DLD (B), Raw264.7 (C), PC12 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of LKB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of LKB1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 6794

Gene ID (Mouse): 20869

Gene ID(Rat):

SwissProt (Human): Q15831

SwissProt (Mouse): Q9WTK7

SwissProt (Rat): D4AE59

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.