Purified Rabbit anti-Leptin Antibody

SKU: DL98741A

Size 1: 100 ul

Synonyms : OB; OBS; Leptin; Obese protein; Obesity factor

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IH

Background: Rabbit polyclonal antibody to Leptin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Leptin. The exact sequence is proprietary.

WB description: Western blot analysis of Leptin expression in LO2 (A), HCT116 (B), MCF7 (C), A549 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Leptin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 3952

Gene ID (Mouse): 16846

Gene ID(Rat):

SwissProt (Human): P41159

SwissProt (Mouse): P41160

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.