Purified Rabbit anti-KIF3A Antibody

SKU: DL96295A

Size 1: 100 ul

Synonyms : KIF3; Kinesin-like protein KIF3A; Microtubule plus end-directed kinesin motor 3A

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to KIF3A

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human KIF3A

WB description: Western blot analysis of KIF3A expression in SW480 (A), SKOV3 (B), mouse brain (C), mouse ovary (D), rat liver (E) whole cell lysates.

IHC description: Immunohistochemical analysis of KIF3A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of KIF3A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 11127

Gene ID (Mouse): 16568

Gene ID(Rat):

SwissProt (Human): Q9Y496

SwissProt (Mouse): P28741

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.