Purified Rabbit anti-HOXC6 Antibody

SKU: DL93010A

Size 1: 100 ul

Synonyms : HOX3C; Homeobox protein Hox-C6; Homeobox protein CP25; Homeobox protein HHO.C8; Homeobox protein Hox-3C

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk, S

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to HOXC6

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HOXC6. The exact sequence is proprietary.

WB description: Western blot analysis of HOXC6 expression in K562 (A), mouse brain (B), rat muscle (C) whole cell lysates.

IHC description: Immunohistochemical analysis of HOXC6 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of HOXC6 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 3223

Gene ID (Mouse): 15425

Gene ID(Rat):

SwissProt (Human): P09630

SwissProt (Mouse): P10629

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.