Purified Rabbit anti-HMGN2 Antibody

SKU: DL93674A

Size 1: 100 ul

Synonyms : HMG17; Non-histone chromosomal protein HMG-17; High mobility group nucleosome-binding domain-containing protein 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R, D, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to HMGN2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HMGN2. The exact sequence is proprietary.

WB description: Western blot analysis of HMGN2 expression in HL60 (A), K562 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of HMGN2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of HMGN2 staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 3151

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P05204

SwissProt (Mouse):

SwissProt (Rat): P18437

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.