Purified Rabbit anti-Histone H2A.X Antibody

SKU: DL95660A

Size 1: 100 ul

Synonyms : H2AX; Histone H2AX; H2a/x; Histone H2A.X

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Histone H2A.X

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of mouse Histone H2A.X. The exact sequence is proprietary.

WB description: Western blot analysis of Histone H2A.Z expression in rat brain (A), mouse brain (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone H2A.Z staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Gene ID(Human):

Gene ID (Mouse): 51788

Gene ID(Rat): 58940

SwissProt (Human):

SwissProt (Mouse): P0C0S6

SwissProt (Rat): P0C0S7

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.