Purified Rabbit anti-HIF1 beta Antibody

SKU: DL93335A

Size 1: 100 ul

Synonyms : BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R, B, P, Rb

Applications: WB, IHC

Background: Rabbit polyclonal antibody to HIF1 beta

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HIF1 beta. The exact sequence is proprietary.

WB description: Western blot analysis of HIF1 beta expression in HeLa (A), U2OS (B), rat muscle (C) whole cell lysates.

IHC description: Immunohistochemical analysis of HIF1 beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 405

Gene ID (Mouse): 11863

Gene ID(Rat): 25242

SwissProt (Human): P27540

SwissProt (Mouse): P53762

SwissProt (Rat): P41739

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.