Purified Rabbit anti-Fibronectin Antibody

SKU: DL92222A

Size 1: 100 ul

Synonyms : FN; Fibronectin; FN; Cold-insoluble globulin; CIG

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, Rb

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Fibronectin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Fibronectin. The exact sequence is proprietary.

WB description: Western blot analysis of Fibronectin expression in HeLa (A), SP2/0 (B), rat lung (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Fibronectin staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 2335

Gene ID (Mouse): 14268

Gene ID(Rat): 14268

SwissProt (Human): P02751

SwissProt (Mouse): P11276

SwissProt (Rat): P04937

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.