Purified Rabbit anti-EPHB1/2 (pY594/604) Antibody

SKU: DL92479A

Size 1: 100 ul

Synonyms : EPHB1; ELK; EPHT2; HEK6; NET; Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2; EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; Ephrin type-B receptor 2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5; EK5; hEK5; Renal carcinoma antigen NY-REN-47; Tyrosine-protein kinase TYRO5; Tyrosine-protein kinase receptor EPH-3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to EPHB1/2 (pY594/604)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPHB1/2 (pY594/604). The exact sequence is proprietary.

WB description: Western blot analysis of EPHB1/2 (pY594/604) expression in A431 (A), HeLa (B), NIH3T3 (C) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of EPHB1/2 (pY594/604) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Gene ID(Human): 2047; 1969

Gene ID (Mouse): 270190

Gene ID(Rat): 24338

SwissProt (Human): P54762; P29323

SwissProt (Mouse): Q8CBF3; P54763

SwissProt (Rat): P09759

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.