Purified Rabbit anti-EIF4E Antibody

SKU: DL90378A

Size 1: 100 ul

Synonyms : EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk, Rb

Applications: WB, IHC

Background: Rabbit polyclonal antibody to EIF4E

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EIF4E. The exact sequence is proprietary.

WB description: Western blot analysis of EIF4E expression in HEK293T (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of EIF4E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 1977

Gene ID (Mouse): 13684

Gene ID(Rat): 117045

SwissProt (Human): P06730

SwissProt (Mouse): P63073

SwissProt (Rat): P63074

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.