Purified Rabbit anti-EIF3L Antibody

SKU: DL92914A

Size 1: 100 ul

Synonyms : EIF3EIP; EIF3S6IP; Eukaryotic translation initiation factor 3 subunit L; eIF3l; Eukaryotic translation initiation factor 3 subunit 6-interacting protein; Eukaryotic translation initiation factor 3 subunit E-interacting protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, B, C, D, Mk, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to EIF3L

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EIF3L. The exact sequence is proprietary.

WB description: Western blot analysis of EIF3L expression in HEK293 (A), A431 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of EIF3L staining in human Pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of EIF3L staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 51386

Gene ID (Mouse): 223691

Gene ID(Rat):

SwissProt (Human): Q9Y262

SwissProt (Mouse): Q8QZY1

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.