Purified Rabbit anti-DNA Polymerase zeta Antibody

SKU: DL93807A

Size 1: 100 ul

Synonyms : POLZ; REV3; DNA polymerase zeta catalytic subunit; Protein reversionless 3-like; REV3-like; hREV3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, D, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to DNA Polymerase zeta

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Polymerase zeta. The exact sequence is proprietary.

WB description: Western blot analysis of DNA Polymerase zeta expression in K562 (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of DNA Polymerase zeta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of DNA Polymerase zeta staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 5980

Gene ID (Mouse): 19714

Gene ID(Rat):

SwissProt (Human): O60673

SwissProt (Mouse): Q61493

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.