Purified Rabbit anti-delta-Sarcoglycan Antibody

SKU: DL97093A

Size 1: 100 ul

Synonyms : Delta-sarcoglycan; Delta-SG; 35 kDa dystrophin-associated glycoprotein; 35DAG

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to delta-Sarcoglycan

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human delta-Sarcoglycan

WB description: Western blot analysis of delta-Sarcoglycan expression in Jurkat (A), THP1 (B), mouse skeletal muscle (C), rat heart (D) whole cell lysates.

IHC description: Immunohistochemical analysis of delta-Sarcoglycan staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of delta-Sarcoglycan staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 6444

Gene ID (Mouse): 24052

Gene ID(Rat):

SwissProt (Human): Q92629

SwissProt (Mouse): P82347

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.